亚博买球

去糖牛奶的制备方法

2016-12-23 18:05:42 admin 110

一种从牛奶或乳清水中提取高纯度蛋白的方法,它采用放置阳离子交换树脂的搅拌罐来吸附、解附、分离提取牛奶或乳清液中的乳铁蛋白、乳氧化酶蛋白及其两者混合物,并辅以膜分离技术将其分离浓缩和高度纯化。搅拌罐可调搅拌浆转速为20-100rpm;罐内侧壁有增涡流挡板,罐底设置带过滤板的树脂床,在树脂床上的树脂层上方8-12cm高度处设置快速导流抽滤管。解决了低成本进行大工业生产的问题。权利要求(10)1、一种从牛奶或乳清水中提取高纯度蛋白的方法,它采用放置阳离子交换树脂的搅拌罐来吸附、解附、分离提取牛奶或乳清液中的乳铁蛋白、乳氧化酶蛋白及其两者混合物,并辅以膜分离技术将其分离浓缩和高度纯化。2、 根据权利要求l所述的方法,其特征是搅拌罐可调搅拌浆转速为20-100rpm;罐内侧壁有增涡流挡板,罐底设置带过滤板的树脂床,在树脂床 上的树脂层上方8-12cm高度处设置快速导流抽滤管。3、 根据权利要求l所述的方法,其特征是罐内设置供自动控制的各种传感 器pH计,重量计,导电率计及温度计,罐内液体保温温度为3-16 0 。C。4..根据权利要求l所述的方法,其特征是树脂为SPC70树脂,树脂吸附解 附的次数和条件可以根据不同产品和产品纯度的要求设定;树脂的吸附时间为 "一180分钟,吸附温度在3-3(TC。5、 根据权利要求l所述的方法,其特征是树脂的吸附时间为35-60分钟。6、 根据权利要求l所述的方法,其特征是树脂的解附釆用两级盐浓度解附 乳铁蛋白、乳氧化酶蛋白。第一级盐浓度为1一2.5%,解附乳氧化酶蛋白,解附 时间为30—10O分钟;第二级盐浓度为3. 5—7%,解附乳铁蛋白,解附时间为30—100 分钟;解附乳氧化酶蛋白及乳铁蛋白混合物的盐浓度为3-7%解附时间为30—100 分钟;解附的搅拌速率为20—100rpm。7、 根据权利要求4所述的方法,其特征是树脂解附所用的盐是钠盐、钙盐, 钾盐;树脂在经吸附和解附工艺过程后,无需再生活化,只要在解附工艺结束 后用反渗透水漂洗树脂至其离子强度低于1.6ms即可供下一批次吸附之用。8、 根据权利要求l所述的方法,其特征是膜分离技术使用O. 05-0. l微米的微滤膜将乳铁蛋白,乳氧化酶蛋白的解附液除菌,去残余的脂肪与其它小颗粒; 用1K-30K分子截流量的超滤膜进行脱盐和浓缩。9、 根据权利要求4所述的方法,其特征是高纯度的乳铁蛋白是将经权利要 求8所述方法吸附解附脱盐浓缩的乳铁蛋白浓液再经过一次弱酸型阳树脂的吸 附解附和超滤膜的浓缩获得的。10、 根据权利要求l所述的方法,其特征是将吸附、解附、脱盐浓缩的乳 铁蛋白浓液,乳氧化酶蛋白浓液及其乳铁蛋白,乳氧化酶蛋白混合浓液进行真 空低温干燥或低温喷雾干燥成粉混合物。说明

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